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1ml×5 PCR Master Mix HS Kit For Nucleic Acid PCR Detection P3082
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | P3082 |
Payment & Shipping Terms:
| Minimum Order Quantity: | 1Bag |
|---|---|
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
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Detail Information |
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| Cat. No.: | P3082 | Specification: | P3082 1ml×5 |
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| Appearance: | Blue Buffer | Group: | PCR Master Mix |
| Activity: | Pass | Amplification Capability: | Good |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | 1ml PCR Master Mix,GDSBio PCR Master Mix,1ml HS Kit |
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Product Description
HSTM Kit
#P3082, 5 ml
Contents:
| 2× HSTM Reaction Mix | 1 ml × 5 |
| HSTM Taq DNA Polymerase (2.5U/μl) | 160 μl |
Store at -20°C
For research use only.
In total 6 vials.
Description
HSTM Kit contains HSTM Taq DNA Polymerase and a premixed, ready-to-use HSTM Reaction Mix, which contains dNTPs, Mg2+ and other components at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. The reaction mix contributes to high specificity by optimizing the system, reducing primer-dimer rate. PCR amplification sensitivity is controllable due to the amount of DNA polymerase is flexible.
HSTM Taq DNA Polymerase is a thermostable recombinant DNA Polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. HSTM Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 2kb/min. It has 5' to 3' polymerase activity, but lacks of 3' to 5' exonuclease activity that results in 3'-dA overhangs PCR products. All components of the HSTM Mix are at optimal concentration for efficient amplification. It contributes to highly specific incorporation of primers and template.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Storage Buffer
20mM Tris-HCl (pH8.0), 1mM DTT, 100mM KCl, 3mM MgCl2, 0.1% NP-40, 0.1% TW 20, 0.2mg/ml BSA, 50% (V/V) Glycerol
Composition of the 2× HSTM Reaction Mix
2× HSTM PCR Buffer, 0.4mM dNTPs, 3.2mM MgSO4, 0.02% bromophenol blue.
HSTM Reaction Mix is a proprietary formulation optimized for robust performance in PCR.
Applications
• High throughput PCR
• High specificity PCR
• Routine PCR with high reproducibility
• Generation of PCR products for TA cloning
Features
• Convenient: only primers and template DNA are added
when prepare final PCR
• High yields of PCR products with minimal optimization
• High efficiency: saving your time by simplifying the process
• Reproducible: lower contamination and pipetting error risk
• High sensitivity: compared to conventional Taq DNA Polymerase
• Flexible: the amount of polymerase is flexible and controllable.
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.