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Long Taq DNA Polymerase #P1063 1,000U

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: P1063
Minimum Order Quantity: 1Bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: P1063 Concentration: 5U/μl
Appearance: Blue Buffer Group: PCR Master Mix
Activity: Pass Amplification Capability: Good
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C

Product Description

Long Taq DNA Polymerase

#P1063, 1,000U

 

Concentration: 5U/μl

Contents:

Long Taq DNA Polymerase 200 μl
10× Long PCR Buffer Ⅰ (Mg2+ Plus) 1.25 ml × 2
10× Long PCR Buffer Ⅱ (Mg2+ Plus) 1.25 ml × 2
PCR Enhancer 500 μl × 2
6× Loading Buffer 1 ml

 

 

Store at -20°C

For research use only.

In total 8 vials.

 

Description

Long Taq DNA Polymerase, a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying long DNA fragments. This specially formulated Long Taq DNA Polymerase was shown to amplify long DNA templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.

Long Taq DNA Polymerase is suitable as a replacement for ordinary Taq Polymerase in most applications. The elongation rate is 3kb/min. The products of Long Taq DNA Polymerase is a mix of 3´-dA overhangs and blunt-ends products, which can be used in TA clone.

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

20mM Tris-HCl (pH 8.0), 100mM KCl, 3mM MgCl2, 1mM DTT, 0.1% NP-40, 0.1% Tween20, 0.2mg/ml BSA, 50% (V/V) glycerol.

10× Long PCR Buffer with Mg2+

500mM Tris-HCl (pH 8.8), 160mM (NH4)2SO4, 25mM MgCl2, 1% Triton X-100.

10× Long PCR Buffer with Mg2+

200mM Tris-HCl (pH 8.8), 100mM KCl, 100mM (NH4)2SO4, 16mM MgSO4, 1% Triton X-100.

Note:

10× Long PCR Buffer Ⅰ is classical Long Taq DNA Polymerase buffer, and it’s good for long template DNA, especially above 10 kb.

10× Long PCR Buffer Ⅱ is a special buffer optimized by Dongsheng Biotech. It is for better fidelity but not good at long template DNA above 10 kb.

Users could choose suitable buffer for different template.

Features

• High fidelity: three times fidelity of Taq DNA Polymerase.

• Longer fragment: amplify long templates as long as 20 kb.

• Amplification of complex template (GC rich or repetitive

sequence).

• Generates 3'-dA and blunt-end PCR products.

Applications

• PCR amplification of complex template DNA

• PCR amplification of long DNA sequences

• DNA sequencing

• PCR for cloning

Long Taq DNA Polymerase #P1063 1,000U 0

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

Long Taq DNA Polymerase #P1063 1,000U 1

Long Taq DNA Polymerase #P1063 1,000U 2

Contact Details
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Phone Number : +8618898478663