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Heat Labile UDG anti-contamination enzyme R5002

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: R5002
Minimum Order Quantity: 1Bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: R5002 Concentration: 1 U/μl
Specification: 100 μl Appearance: Colourless
Application: Molecular Biology Classification: General Reagents
Grade: Molecular Biology Logo Printing: With Logo Printing
Transport Package: Packing Production Capacity: 100 Bag/Bags Per Day
Storage Conditions: Store At -20°C

Product Description

Heat Labile UDG

For research use only

Components

Component R5001 (500 U) R5002 (100 U)
Heat Labile UDG (1 U/μl) 500 μl 100 μl

 

Storage

This reagent should be kept at -20°C.

 

Description

Heat Labile UDG is purified from the expression product of an recombinant E. coli strain containing the UDG gene cloned from cold-adaptive marine bacteria (psychrophilic Marine bacterium). UDG (Uracil-DNA Glycosylase) catalyzes the hydrolysis of uracil bases of the single or double stranded DNA containing dU and the N-glycoside bonds of the glycosphosphoric acid skeleton to release free uracil. The resulting base-free site is easily fractured by hydrolysis. This product is sensitive to high temperature, and above 50°C can make the enzyme irreversible inactivation, suitable for PCR, qPCR, RT-PCR, RT-qPCR system.

 

Storage Buffer

20 mM Tris-HCl, pH 7.5 at 25°C

0.1 mM EDTA

100 mM KCl

1 mM DTT

Glycerol 50% (v/v)

CA630 0.5% (v/v)

0.5% Tween - 20 (v/v)

 

Source

Psychrophilic Marine Bacteium derived Heat Labile UDG expressed and purified in E. coli.

 

Unit Definition

In 70 mM Tris-HCl, pH 7.5, 10 mM NaCl, 1 mM EDTA, 100 g/ml BSA reaction solution, the amount of enzyme required to release 1 nmol uracil from DNA containing dU within 1 h at 37°C was defined as 1 unit of activity (U).

 

Thermal inactivation

95°C, 2 min

 

Heat Labile UDG  anti-contamination enzyme R5002 0

Heat Labile UDG  anti-contamination enzyme R5002 1

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

Heat Labile UDG  anti-contamination enzyme R5002 2

Heat Labile UDG  anti-contamination enzyme R5002 3

Contact Details
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Phone Number : +8618898478663