【Product Name】

Fast DNA Library Prep Kit for MGI

【Cat. No./Spec.】

KM001-A/24 rxns; KM001-B/96 rxns; sample sack/ 6 rxns

【Product Description】

Aiming at MGI high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, and reagents are highly premixed, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

【Sample Type】

Application Sample Type Recommended Amount

Whole genome sequencing High quality complex genomes 50ng-1μg

Target capture sequencing of whole exome High quality complex genomes 10ng-1μg

Target capture sequencing of whole genome FFPE DNA ≥50ng

Target capture sequencing of whole genome cfDNA/ctDNA ≥100pg

Whole genome sequencing Microbial genome 1ng-1μg

ChIP-Seq ChIP DNA ≥100pg

Targeted sequencing Amplicon ≥100pg

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.

【Components】

Component 24 rxns 96 rxns

End Repair & A-Tailing Mix 480 μl 4×480 μl

Fast DNA Ligase 120 μl 2×240 μl

Fast Ligation Buffer 600 μl 4×600 μl

2× HIFI Library PCR Master Mix 600 μl 4×600 μl

Primer Mix for MGI* 120 μl 480 μl

* If there are more than one sample, #KM002 and #KM003 adapter primer mix is recommended. This kit provides a set of primers.

Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.