Fast DNA Library Plus Prep Kit for MGI

Fast DNA Library Plus Prep Kit for MGI

Product Details:

Place of Origin: China
Brand Name: GDSBio
Certification: ISO9001, ISO13485

Payment & Shipping Terms:

Minimum Order Quantity: 1 box
Price: $67.20/ box 1 box
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100000 rxns/Kit per Day
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Detail Information

Model Number: KM004-A, KM004-B Spec.: KM004-A/24 Rxns; KM004-B/96 Rxns
Application: NGS Library Construction Sample Type: MGI
Logo Printing: With Logo Printing Transport Package: Packing
Storage Conditions: Store At -20°C Shelf Life: 12 Months
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Product Description

Fast DNA Library Plus Prep Kit for MGI

Instruction for Use

【Product Name】

Fast DNA Library Plus Prep Kit for MGI

【Cat. No./Spec.】

KM004-A/24 rxns; KM004-B/96 rxns; sample sack/ 6 rxns

【Product Description】

Aiming at MGI high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines fragmentation, end repair and A-Tailing into one step, greatly shortening the time of library construction and reducing the error caused by tedious steps. After fragmentation and end preparation, the product can be directly ligated with adapter without additional purification, and the subsequent procedure is the same as that of #KM001 Fast DNA Library Prep Kit for MGI. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

【Sample Type】

Table 1 Recommended Inputs for Common DNA

Application Sample Type Recommended Amount
Whole genome sequencing High quality complex genomes 50ng-1μg
Target capture sequencing of whole exome High quality complex genomes 10ng-1μg
Target capture sequencing of whole genome FFPE DNA ≥50ng
Whole genome sequencing Microbial genome 1ng-1μg

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.


Component 24 rxns 96 rxns
FEP Buffer 120 μl 480 μl
FEP Enzyme Mix 240 μl 2×480 μl
Fast DNA Ligase 120 μl 2×240 μl
Fast Ligation Buffer 600 μl 4×600 μl
2× HIFI Library PCR Master Mix 600 μl 4×600 μl
Primer Mix for MGI * 120 μl 480 μl
Neutralization Buffer 120 μl 480 μl

*FEP Buffer is the fragmentation and end preparation reaction buffer. FEP Enzyme Mix is a mixture of enzyme related to fragmentation and end preparation.

* If there are more than one sample, #KM002 and #KM003 adapter primer mix is recommended. This kit provides a set of primers, the primer sequence is as follows:



Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.


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Fast DNA Library Plus Prep Kit for MGI 1


GDSBio Is a high-tech enterprise focusing on research and development, production and sales of high-quality life science products. The company has a complete product line, with PCR technology as the core, focusing on general PCR, fluorescence quantitative PCR, NGS storage, nucleic acid electrophoresis and other molecular biology technologies, and has developed molecular research reagents, molecular in vitro diagnostic raw materials, nucleic acid extraction and detection reagents and other products.

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