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150ul 300ul Lyticase Enzyme N9032 Colourless Solution
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | N9032 |
Payment & Shipping Terms:
| Minimum Order Quantity: | 1 bag |
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| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
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Detail Information |
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| Cat. No.: | N9032 | Size: | 150/300ul |
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| Concentration: | 10U/µl | Appearance: | Colourless Solution |
| Group: | In Vitro Diagnostic Products | Recommend Use Concentration: | 0.15U/μl |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | 300ul Lyticase Enzyme,150ul Lyticase Enzyme,lyticase |
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Product Description
Lyticase
Components
| Component | N9031 | N9032 |
| Lyticase | 150ul | 300ul |
| Buffer | 10ml | 20ml |
Recommend use concentration: 0.15U/μl
Store at -20°C
For research use only
Description
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Applications
- Digest yeast cell walls
- Generate spheroplasts from fungi for transformation
Biochem/physiol Actions
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Definition of Activity Unit
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25°C, using a suspension of yeast as substrate in a 3 ml reaction mixture.
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