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V5001 One Step Rt PCR Master Mix

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: V5001
Minimum Order Quantity: 1Bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: V5001 Specification: 200 Rxn, 30 μl/rxn
Appearance: Colourless Application: RNA Reverse Transcription And Quantitative PCR
Activity: Pass Amplification Capability: Good
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
High Light:

V5001 one step rt pcr master mix

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200 rxn one step rt pcr master mix

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200 rxn rt pcr mix


Product Description

One-step Probe RT-qPCR Kit

For research use only

Components

Component V5001 (200 rxn, 30 μl/rxn)
2 × One-step U+ Mix a 760 μl × 4
One-step U+ Enzyme Mix b 308 μl
RNase-free ddH2O 800 μl × 4
50 × ROX Reference Dye 1 c 120 μl
50 × ROX Reference Dye 2 c 120 μl

a. Contains dNTP/dUTP Mix, Mg2+.

b. Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and hot-start Taq DNA Polymerase.

c. Used to correct the fluorescence signal error between holes. 50 × ROX Reference Dye 1 is used for ABI 7900HT/ 7300 real-time PCR System and StepOne Plus; ABI 7500, 7500 Fast real-time PCR System, Stratagene Mx3000P using 50 × ROX Reference Dye 2; Roche, bio-rad Real Time PCR Systems do not have to use ROX.

 

Storage

This reagent should be kept at -20°C.

 

Description

The One-step Probe RT-qPCR Kit is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 2 × One-step U+ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan®. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

 

Applications

• Probe gene expression analysis

• Probe Low-copy gene detection

• Probe microarray validation

• Probe gene knockdown validation

 

Features

• One-step RT-qPCR

• This kit is suitable for fluorescence quantification by probe method

• This kit is compatible with many real-time systems

• Hot-start technology brings high specificity and reproducible amplification

• dUTP/UDG system, effectively prevent PCR product contamination

V5001 One Step Rt PCR Master Mix 0

V5001 One Step Rt PCR Master Mix 1

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

V5001 One Step Rt PCR Master Mix 2

V5001 One Step Rt PCR Master Mix 3

Contact Details
sales

Phone Number : +8618898478663