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Colourless Appearance M-MLV Reverse Transcriptase In PCR R1041

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: R1041
Minimum Order Quantity: 1Bag
Price: US $19.9-59.9 / Bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: R1041/R1042 Concentration: 200U/μl
Appearance: Colourless Group: Reverse Transcriptase PCR Reagents
Activity: Pass Amplification Capability: /
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
High Light:

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Product Description

M-MLV Reverse Transcriptase

M-MLV Reverse Transcriptase Rt-PCR R1041

For research use only

 

Components of the kit

Component R1041 (5,000U) R1042 (10,000U)
M-MLV (200U/μl) 25 μl 50μl
5× First-strand Buffer 100μl 200 μl

 

Description
The Dongsheng M-MLV Reverse Transcriptase (RT) is a genetically modified M-MLV RT. It differs from the M-MLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a ribonuclease H activity specific to RNA in RNA-DNA hybrids. Dongsheng M-MLV Reverse Transcriptase has significantly lower RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Source
E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

Applications
Generation of first strand cDNA for use in:
-PCR, see Protocol for First-strand cDNA Synthesis;
-real-time PCR;
-second strand cDNA synthesis;
DNA labeling;
Analysis of RNA by primer extension.

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests.

Functionally tested in first strand cDNA synthesis.

Store Buffer
20m M Tris-HCl (pH 7.5)
200 mM NaCl
0.1 mM EDTA
1 mM DTT
0.01% NP-40
50% glycerol

5xFirst-Strand Buffer
250 mM Tris-HCl (pH 8.3 at 25°C)
375 mM KCl
15 mM MgCl2
50 mM DTT

 

M-Mlv Reverse Transcriptase Rt-PCRM-Mlv Reverse Transcriptase Rt-PCR

M-Mlv Reverse Transcriptase Rt-PCR
Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.

M-Mlv Reverse Transcriptase Rt-PCR

Contact Details
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Phone Number : +8618898478663