Aiming At MGI High Throughput Sequencing Platform Fast DNA NGS Library Plus Prep Kit

Aiming At MGI High Throughput Sequencing Platform Fast DNA NGS Library Plus Prep Kit

Product Details:

Place of Origin: China
Brand Name: GDSBio
Certification: ISO9001, ISO13485
Model Number: KM004-A, KM004-B

Payment & Shipping Terms:

Minimum Order Quantity: 1 Kit
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 10000 Tets per Day
Get Best Price Contact Now

Detail Information

Cat. No.: KM004-A, KM004-B Specification: KM004-A/24 Rxns; KM004-B/96 Rxns
Appearance: Complete, No Damage Stock: Yes
Technology: Next Generation Sequencing Platform: MGI
Logo Printing: With Logo Printing Transport Package: Packing
Shelf Life: 12 Months Storage Conditions: Stored At -20°C
High Light:

NGS Library Plus Prep Kit

,

Fast DNA Library Plus Prep Kit

Product Description

Fast DNA Library Plus Prep Kit for MGI

【Product Name】

Fast DNA Library Plus Prep Kit for MGI

【Cat. No./Spec.】

KM004-A/24 rxns; KM004-B/96 rxns; sample sack/ 6 rxns

【Product Description】

Aiming at MGI high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines fragmentation, end repair and A-Tailing into one step, greatly shortening the time of library construction and reducing the error caused by tedious steps. After fragmentation and end preparation, the product can be directly ligated with adapter without additional purification, and the subsequent procedure is the same as that of #KM001 Fast DNA Library Prep Kit for MGI. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

【Sample Type】

Table 1 Recommended Inputs for Common DNA

Application Sample Type Recommended Amount
Whole genome sequencing High quality complex genomes 50ng-1μg
Target capture sequencing of whole exome High quality complex genomes 10ng-1μg
Target capture sequencing of whole genome FFPE DNA ≥50ng
Whole genome sequencing Microbial genome 1ng-1μg

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.

【Components】

Component 24 rxns 96 rxns
FEP Buffer 120 μl 480 μl
FEP Enzyme Mix 240 μl 2×480 μl
Fast DNA Ligase 120 μl 2×240 μl
Fast Ligation Buffer 600 μl 4×600 μl
2× HIFI Library PCR Master Mix 600 μl 4×600 μl
Primer Mix for MGI * 120 μl 480 μl
Neutralization Buffer 120 μl 480 μl

*FEP Buffer is the fragmentation and end preparation reaction buffer. FEP Enzyme Mix is a mixture of enzyme related to fragmentation and end preparation.

* If there are more than one sample, #KM002 and #KM003 adapter primer mix is recommended. This kit provides a set of primers.

Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.

Aiming At MGI High Throughput Sequencing Platform Fast DNA NGS Library Plus Prep Kit 0Aiming At MGI High Throughput Sequencing Platform Fast DNA NGS Library Plus Prep Kit 1

 

Want to Know more details about this product
I am interested in Aiming At MGI High Throughput Sequencing Platform Fast DNA NGS Library Plus Prep Kit could you send me more details such as type, size, quantity, material, etc.
Thanks!
Waiting for your reply.