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Heat Labile Anti Contamination UDG Enzyme Molecular Biology For QPCR R5001 1 U/μL
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | R5001 |
Payment & Shipping Terms:
| Minimum Order Quantity: | 1Bag |
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| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
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Detail Information |
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| Cat. No.: | R5001 | Concentration: | 1 U/μl |
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| Specification: | 500 μl | Appearance: | Colourless |
| Application: | Molecular Biology | Classification: | General Reagents |
| Grade: | Molecular Biology | Logo Printing: | With Logo Printing |
| Transport Package: | Packing | Production Capacity: | 100 Bag/Bags Per Day |
| Storage Conditions: | Store At -20°C | ||
| High Light: | R5001 Anti Contamination Enzyme,Molecular Biology Heat Labile UDG,1 U/ΜL Anti Contamination UDG Enzyme |
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Product Description
Heat Labile UDG
For research use only
Heat Labile UDG Highly effective anti-contamination enzyme R5001
Components
| Component | R5001 (500 U) | R5002 (100 U) |
| Heat Labile UDG (1 U/μl) | 500 μl | 100 μl |
Storage
This reagent should be kept at -20°C.
Description
Heat Labile UDG is purified from the expression product of an recombinant E. coli strain containing the UDG gene cloned from cold-adaptive marine bacteria (psychrophilic Marine bacterium). UDG (Uracil-DNA Glycosylase) catalyzes the hydrolysis of uracil bases of the single or double stranded DNA containing dU and the N-glycoside bonds of the glycosphosphoric acid skeleton to release free uracil. The resulting base-free site is easily fractured by hydrolysis. This product is sensitive to high temperature, and above 50°C can make the enzyme irreversible inactivation, suitable for PCR, qPCR, RT-PCR, RT-qPCR system.
Storage Buffer
20 mM Tris-HCl, pH 7.5 at 25°C
0.1 mM EDTA
100 mM KCl
1 mM DTT
Glycerol 50% (v/v)
CA630 0.5% (v/v)
0.5% Tween - 20 (v/v)
Source
Psychrophilic Marine Bacteium derived Heat Labile UDG expressed and purified in E. coli.
Unit Definition
In 70 mM Tris-HCl, pH 7.5, 10 mM NaCl, 1 mM EDTA, 100 g/ml BSA reaction solution, the amount of enzyme required to release 1 nmol uracil from DNA containing dU within 1 h at 37°C was defined as 1 unit of activity (U).
Thermal inactivation
95°C, 2 min
GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, nucleic acid electrophoresis, nucleic acid purification, it has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.