|Place of Origin:||China|
|Minimum Order Quantity:||1Bag|
|Packaging Details:||small package or bulk distribute or OEM|
|Delivery Time:||8 work days|
|Payment Terms:||L/C, D/A, D/P, T/T, Western Union, MoneyGram|
|Supply Ability:||100 Bag/Bags Per Day|
|Cat. No.:||P1111/P1112/P1113||Concentration:||8,000 U/mL|
|Appearance:||Blue Buffer||Group:||PCR Master Mix|
|Logo Printing:||With Logo Printing||Transport Package:||Packing|
|Production Capacity:||100 Bag/Bags Per Day||Storage Conditions:||Store At -20°C|
P1113 PCR Master Mix,
8000 U/mL PCR Master Mix
Bst DNA Polymerase, Exonuclease Minus
|Sizes||1 x 25 µL||1 x 250 µL||5 x 250 µL|
Includes 10X DNA Polymerase Buffer B (1.2 ml per 2,000 Units)
Store at –20 ºC.
For Research Use Only. Not for use in Diagnostic Procedures.
Bst DNA Polymerase, Exonuclease Minus, 8,000 units/mL.
10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, and 50% Glycerol.
Bst DNA Polymerase, Exonuclease Minus is stable for one year from the date received if stored at –20 ºC.
Recommended Reaction Conditions
8 U Bst DNA Polymerase, Exonuclease Minus; 1X DNA Polymerase Buffer B containing 20 mM Tris-HCl pH 8.8, 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4,and 0.1 % Triton X-100.
One unit catalyzes the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65 °C in 20 mM Tris-HCl pH 8.8 , 10 mM (NH4)2SO4,10 mM KCl, 2 mM MgSO4, 0.1 % Triton X-100, 30 nM M13mp18 ssDNA, 70 nM M13 sequencing primer(-47) 24 mer, 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP), and 0.1 mg/mL BSA.
Absence of Endonuclease or Nicking Activity
Incubation of 8 U of Bst DNA Polymerase, Exonuclease Minus with 1 µg of pUC19 DNA for 16-18 hours at 37 ºC resulted in no smearing of bands as detected by agarose gel electrophoresis.
Absence of Exonuclease Activity
Incubation of 8 U of Bst DNA Polymerase, Exonuclease Minus with 1 µg of HindIII-cut lambda DNA for 16 hours at 37 ºC and 65 ºC resulted in no smearing of bands on agarose gels. Single stranded and double stranded exonuclease activities were tested by incubating 10 µL of enzyme at 8 U/ µLwith radiolabeled DNA substrate for one hour at 37 ºC and 65 ºC, resulting in less than 0.1% release of TCA-soluble counts.
>90% pure by SDS PAGE. No detectable DNA contamination. 10 µL of enzyme at 8 U/ µL of the sample was tested for E. coli genomic DNA contamination by PCR amplifying with the E. coli 16S ribosomal primers.
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.