High Throughput Sequencing Platform NGS Library Construction Fast DNA Plus Prep Kit K004-A

High Throughput Sequencing Platform NGS Library Construction Fast DNA Plus Prep Kit K004-A

Product Details:

Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: K004-A

Payment & Shipping Terms:

Minimum Order Quantity: 1Bag
Price: /
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Get Best Price Contact Now

Detail Information

Cat. No.: K004-A Concentration: 24 Rxns
Appearance: Colourless Group: NGS Library Construction
Activity: Pass Amplification Capability: Good
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
High Light:

K004-A NGS Library Construction

,

Fast DNA NGS Library Construction

Product Description

【Product Name】

Fast DNA Library Plus Prep Kit

【Cat. No./Spec.】

K004-A/24 rxns; K004-B/96 rxns; sample sack/ 6 rxns

【Product Description】

Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines fragmentation, end repair and end “A” tailing into one step, greatly shortening the time of library construction and reducing the error caused by tedious steps. After fragmentation and end repair, the product can be directly ligated with adapter without additional purification, and the subsequent procedure is the same as that of #K001 Fast DNA Library Prep Kit. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

【Sample Type】

Table 1 Recommended Inputs for Common DNA

Application Sample Type Recommended Amount
Whole genome sequencing High quality complex genomes 50ng-1μg
Target capture sequencing of whole exome High quality complex genomes 10ng-1μg
Target capture sequencing of whole genome FFPE DNA ≥50ng
Whole genome sequencing Microbial genome 1ng-1μg
Whole genome sequencing(PCR-free) High quality DNA

≥50ng (no size selection)

≥200ng (size selection)

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.

【Components】

Component 24 rxns 96 rxns
FEP Buffer 120 μl 480 μl
FEP Enzyme Mix 240 μl 2×480 μl
Fast DNA Ligase 120 μl 2×240 μl
Fast Ligation Buffer 600 μl 4×600 μl
2× HIFI Library PCR Master Mix 600 μl 4×600 μl
Primer Mix* 120 μl 480 μl
Neutralization Buffer 120 μl 480 μl

*FEP Buffer is the fragmentation and end repair reaction buffer. FEP Enzyme Mix is a mixture of enzyme related to fragmentation and end repair.

* If there are more than one sample, #K002 and #K003 adapter primer mix is recommended. 

Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.

High Throughput Sequencing Platform NGS Library Construction Fast DNA Plus Prep Kit K004-A 0

High Throughput Sequencing Platform NGS Library Construction Fast DNA Plus Prep Kit K004-A 1

M-Mlv Reverse Transcriptase Rt-PCR
Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.

High Throughput Sequencing Platform NGS Library Construction Fast DNA Plus Prep Kit K004-A 3

Want to Know more details about this product
I am interested in High Throughput Sequencing Platform NGS Library Construction Fast DNA Plus Prep Kit K004-A could you send me more details such as type, size, quantity, material, etc.
Thanks!
Waiting for your reply.