Whole Genome Sequencing NGS Library Construction Fast DNA Library Prep Kit V2 K001S-A K001S-B
|Place of Origin:||China|
Payment & Shipping Terms:
|Minimum Order Quantity:||1Bag|
|Packaging Details:||small package or bulk distribute or OEM|
|Delivery Time:||8 work days|
|Payment Terms:||L/C, D/A, D/P, T/T, Western Union, MoneyGram|
|Supply Ability:||100 Bag/Bags Per Day|
|Cat. No.:||K001S-A/K001S-B||Concentration:||K001S-A/24 Rxns; K001S-B/96 Rxns; Sample Sack/ 6 Rxns|
|Appearance:||Colourless||Group:||NGS Library Construction|
|Logo Printing:||With Logo Printing||Transport Package:||Packing|
|Production Capacity:||100 Bag/Bags Per Day||Storage Conditions:||Store At -20°C|
Whole Genome Sequencing NGS Library Construction,
Fast DNA NGS Library Construction
Fast DNA Library Prep Kit V2
Fast DNA Library Prep Kit V2
K001S-A/24 rxns; K001S-B/96 rxns; sample sack/ 6 rxns
Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.
|Application||Sample Type||Recommended Amount|
|Whole genome sequencing||High quality complex genomes||50ng-1μg|
|Target capture sequencing of whole exome||High quality complex genomes||10ng-1μg|
|Target capture sequencing of whole genome||FFPE DNA||≥50ng|
|Target capture sequencing of whole genome||cfDNA/ctDNA||≥100pg|
|Whole genome sequencing||Microbial genome||1ng-1μg|
|Whole genome sequencing(PCR-free)||High quality DNA||
≥50ng (no size selection)
≥200ng (size selection)
【Storage Condition & Shelf Life】
All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.
|Component||24 rxns||96 rxns|
|End Repair & A-Tailing Enzyme Mix||120 μl||2×240 μl|
|End Repair & A-Tailing Buffer||240 μl||2×480 μl|
|Fast DNA Ligase||120 μl||2×240 μl|
|Fast Ligation Buffer||600 μl||4×600 μl|
|2× HIFI Library PCR Master Mix||600 μl||4×600 μl|
|Primer Mix*||120 μl||480 μl|
* If there are more than one sample, #K002 and #K003 adapter primer mix is recommended. This kit provides a set of primers with index, the primer sequence is as follows.
Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.
GDSBio, we specialize in the research and development, production, and sales of molecular biology reagents. Our product line includes a variety of reagents for molecular biology research and diagnosis, covering ordinary PCR, fluorescence quantitative PCR, NGS library preparation, nucleic acid electrophoresis, and more.