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One Tube NGS Library Construction Fast DNA Library Prep Kit For MGI KM001-A KM001-B
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | KM001-A/KM001-B |
Payment & Shipping Terms:
| Minimum Order Quantity: | 1Bag |
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| Price: | / |
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
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Detail Information |
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| Cat. No.: | KM001-A/KM001-B | Concentration: | KM001-A/24 Rxns; KM001-B/96 Rxns; Sample Sack/ 6 Rxns |
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| Appearance: | Colourless | Group: | NGS Library Construction |
| Activity: | Pass | Amplification Capability: | Good |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | One Tube NGS Library Construction,MGI KM001-B NGS Library Construction |
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Product Description
Fast DNA Library Prep Kit for MGI
【Product Name】
Fast DNA Library Prep Kit for MGI
【Cat. No./Spec.】
KM001-A/24 rxns; KM001-B/96 rxns; sample sack/ 6 rxns
【Product Description】
Aiming at MGI high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, and reagents are highly premixed, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.
【Sample Type】
| Application | Sample Type | Recommended Amount |
| Whole genome sequencing | High quality complex genomes | 50ng-1μg |
| Target capture sequencing of whole exome | High quality complex genomes | 10ng-1μg |
| Target capture sequencing of whole genome | FFPE DNA | ≥50ng |
| Target capture sequencing of whole genome | cfDNA/ctDNA | ≥100pg |
| Whole genome sequencing | Microbial genome | 1ng-1μg |
| ChIP-Seq | ChIP DNA | ≥100pg |
| Targeted sequencing | Amplicon | ≥100pg |
【Storage Condition & Shelf Life】
All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.
【Components】
| Component | 24 rxns | 96 rxns |
| End Repair & A-Tailing Mix | 480 μl | 4×480 μl |
| Fast DNA Ligase | 120 μl | 2×240 μl |
| Fast Ligation Buffer | 600 μl | 4×600 μl |
| 2× HIFI Library PCR Master Mix | 600 μl | 4×600 μl |
| Primer Mix for MGI* | 120 μl | 480 μl |
* If there are more than one sample, #KM002 and #KM003 adapter primer mix is recommended. This kit provides a set of primers, the primer sequence is as follows:
5’-TGTGAGCCAAGGAGTTG-3’
5’-GAACGACATGGCTACGA-3’
Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.
GDSBio, we specialize in the research and development, production, and sales of molecular biology reagents. Our product line includes a variety of reagents for molecular biology research and diagnosis, covering ordinary PCR, fluorescence quantitative PCR, NGS library preparation, nucleic acid electrophoresis, and more.
Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.