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T4 Polynucleotide Kinase T4 PNK #K013 Logo Printing
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | K013 |
Payment & Shipping Terms:
| Minimum Order Quantity: | 500U |
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| Price: | inquiry |
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
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Detail Information |
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| Cat. No.: | K013-A | Specification: | 500U |
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| Appearance: | Colourless | Application: | 5' Phosphorylation Of DNA/RNA For Subsequent Ligation |
| Classification: | General Reagents | Grade: | Molecular Biology |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | T4 Polynucleotide Kinase,PNK T4 Polynucleotide Kinase |
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Product Description
Cat. No./Spec.: K013-A/500U; K013-B/2500U; K013-C/10000U
Concentration: 10 U/µL
Product Description
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5'-hydroxyl terminus of polynucleotides (double-stranded and single-stranded DNA and RNA) and nucleoside 3'-monophosphate. T4 polynucleotide kinase also catalyzes the removal of 3′-phosphoryl groups from 3′-phosphate-based polynucleotides, deoxynucleoside 3′-monophosphate, and deoxynucleoside 3′-diphosphate. When ADP is present, T4 PNK has 5'-end phosphatase activity, catalyzing the exchange of 5'-P-oligomeric/polymeric nucleotides and ATP terminal 5'-phosphate groups. It is suitable for constructing DNA libraries and producing probes with terminal labeling.
Components
| Component |
K013-A (500 U) |
K013-B (2,500 U) |
K013-C (10,000 U) |
| T4 Polynucleotide Kinase (10 U/µL) | 50 µL | 250 µL | 1 mL |
| 10X T4 PNK Buffer * | 500 µL | 1 mL | 1 mL × 2 |
* 10 × T4 PNK Buffer does not contain ATP, and customers need to add it themselves, with a final concentration of 1 mM, or use T4 DNA ligase buffer.
Storage Condition & Shelf Life
All reagents should be stored at -20°C. The product is valid for 12 months.
Unit Definition
One Richardson unit is defined as the amount of enzyme catalyzing the recombination of 1 nmol of [γ-32P] ATP.
Scope of Application
1. End labeling DNA or RNA for probes and DNA sequencing;
2. 5' phosphorylation of DNA/RNA for subsequent ligation;
3. Phosphorylate the 5´ end of the 3´ phosphorylated mononucleotide to prepare the pNp substrate for addition to the 3´ end of DNA or RNA;
4. Label the 5´ end of oligonucleotides with a phosphate group at the 3´ end.
