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Colourless T4 DNA Ligase (Fast) #K012
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | K012 |
Payment & Shipping Terms:
| Minimum Order Quantity: | 1000U |
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| Price: | inquiry |
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
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Detail Information |
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| Cat. No.: | K012 | Specification: | 1000U |
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| Appearance: | Colourless | Application: | DNA Ligation |
| Classification: | General Reagents | Grade: | Molecular Biology |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | T4 DNA Ligase fast,T4 DNA Ligase |
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Product Description
Cat. No./Spec.: K012/1000 U
Concentration: 5 U/µL
Note: 1 U=1 Weiss unit
Product Description
T4 DNA Ligase (Fast) is produced by E. coli carrying T4 bacteriophage gene 30. This enzyme catalyzes the formation of phosphodiester bonds between the 5'-phosphate group and the 3'-hydroxyl group of double-helix DNA or RNA. This enzyme can repair single-strand breaks in double-stranded DNA, RNA, or DNA/RNA complexes, and can connect DNA fragments with sticky or blunt ends, but it is inactive for single-stranded nucleic acids. It is mainly used for cloning of restriction enzyme digestion products, gene site-directed mutagenesis, PCR product cloning, linear DNA self-looping, and repair of double-stranded DNA breaks. T4 DNA Ligase (Fast) requires ATP as a cofactor and completes the sticky end ligation reaction at room temperature in only 10 minutes.
Components
| Component | K012 (1,000 U) |
| T4 DNA Ligase (Fast) (5 U/µL) | 200 µL |
| 10X T4 DNA Ligase Buffer | 1 mL |
| 50% PEG | 1 mL |
Storage Condition
Store at -20°C.
Unit Definition
At 37°C, 1 Weiss unit of enzyme catalyzes the conversion of 1 nmol of [32PPi] into an activated carbon adsorption state within 20 minutes. One Weiss unit is equivalent to approximately 200 cohesive end ligation units (CEU), which is equivalent to the number of HindIII digested DNA fragments that can be ligated within 30 minutes at 16°C. λ DNA fragments.
