Cat. No: P2101, 400 rxn/20μl reaction
P2102, 2000 rxn/20μl reaction
Power Green qPCR Mix
For research use only
|2X Power Green qPCR Mix
||1 ml × 5
||1 ml × 5
This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.
Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the SYBR Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on.
The Hotstart Taq DNA Polymerase in the mix is chemically modified, and it can reduce non-specific amplification more effectively than the antibody-modified polymerase. At the same time, the mix contains aptamer-based inhibitors, which reduce the generation of the primer dimer and secondary metabolites. When the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This dual hot-start technique brings greater efficiency and specificity to PCR.
• Gene expression analysis
• Low-copy gene detection
• Microarray validation
• Gene knockdown validation
• Compatible with many Real-time systems which not require ROX reference dye
• Exceptional specificity with dual hot-start mechanism
• Tight reproducibility in Ct values over a broad dynamic range
• Universal instrument compatibility