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R2011 1000U RNase Inhibitor In Vitro Transcription
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | R2011 |
Payment & Shipping Terms:
| Minimum Order Quantity: | 1 bag |
|---|---|
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
|
Detail Information |
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| Cat. No.: | R2011 | Concentration: | 40U/μl |
|---|---|---|---|
| Appearance: | Colourless | Group: | Reverse Transcriptase PCR Reagents |
| Activity: | Pass | Amplification Capability: | / |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | 1000U RNase Inhibitor,R2011 RNase Inhibitor,R2011 rnasin inhibitor |
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Product Description
RNasin (RNase Inhibitor) #R2011 1,000U
RNasin (RNase Inhibitor)
Components
| Component | R2011 (1,000 U) |
|
Concentration |
40U/μl |
Store at -20°C
For research only
In total 1 vial.
Description
RNasin is a ribonuclease inhibitor purified from human placenta. RNasin reduces the activity of RNase A-type enzymes in a variety of organisms.
Source
E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor.
Storage Buffer
20mM HEPES-NaOH (pH7.5), 50mM NaCl, 8mM DTT, 0.5mM ELUGENT Detergent and 50% (v/v) glycerol.
Feature
Free of endodeoxyribonucleases, exodeoxyribonucleases, rib- onucleases and phosphatases.
Definition of Activity Unit
One unit of the protein inhibits the activity of 5ng RNase A by 50%.
Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/ml BSA, 100 ng/ml RNase A, 0.1 mg/ml E.coli [3H]-RNA, 50 mg/ml yeast RNA, 8 mM DTT.
Applications
• Inhibition of RNA degradation in the following procedures:
-in vitro transcription;
-cDNA synthesis;
-in vitro translation;
-isolation of mammalian cell fractions that contain mRNA
protein complex.
• Separation and identification of specific ribonuclease activities.
• Studies of tumor suppression.
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