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Colourless Appearance RNase Inhibitor Murine R4001 20000U

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: R4001
Minimum Order Quantity: 1 bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: R4001 Concentration: 20,000U
Appearance: Colourless Group: Reverse Transcriptase PCR Reagents
Activity: Pass Amplification Capability: /
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
High Light:

Colourless Appearance RNase Inhibitor Murine

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20000U RNase Inhibitor Murine

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20000U rnase inhibitor chemical


Product Description

RNase Inhibitor Murine R4001 20,000U

RNase Inhibitor (Murine)

For research use only

Components

Component R4001 (20,000 U)
RNase Inhibitor (Murine) (40 U/μl)

500 μl

 

Storage

This reagent should be kept at -20°C.

 

Description

RNase Inhibitor (Murine) is a recombinant murine source RNase inhibitor expressed in the soluble form in E. coli. It can inhibit a wide range of RNases (RNase A, B, C). It has been tested by RT-PCR, RT-qPCR and is compatible with various reverse transcriptase and DNA polymerases. Compared with human source RNase inhibitors, murine source RNase inhibitors do not contain two of the very oxidation-sensitive cysteines of human proteins, so they have higher antioxidant activity and are more suitable for experiments with high DTT sensitivity, such as qPCR.

 

Unit Definition

The amount of enzyme required to inhibit 50% of 5 ng RNase A activity was defined as 1 unit of activity (U). The activity of RNase A is quantitatively obtained by hydrolyzing 2 ', 3' -CMP to generate 3 '-CMP.

 

Quality Control

Exonuclease residue detection: 200 U of this product and 0.6 μg of λ-Hind III were incubated at 37°C for 16 h, and the DNA bands did not change after electrophoresis.

Detection of endonuclease residue: 200 U of this product and 0.6 μg of Supercoiled pBR322 DNA were incubated at 37°C for 4 h, and the DNA bands did not change after electrophoresis.

E.coli DNA residue detection: the nucleic acid residue in 200 U of this product was detected by E. coli gDNA-specific TaqMan qPCR, and the residue of E. coli genome was less than 10 copies.

 

Application

1. Synthesis of the first strand of cDNA

2. Polysome isolation

3. In vitro transcription

4. No cell translation system in vitro

 

Matters Needing Attention

1. RNase activity was inhibited by a wide range of pH values, with the maximum activity at pH 7 ~ 8.

2. Foaming or vigorous stirring (Vortex, etc.) can cause inactivation.

3. RNase H activity will not be inhibited.


Colourless Appearance RNase Inhibitor Murine R4001 20000U 0

Colourless Appearance RNase Inhibitor Murine R4001 20000U 1

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