|Place of Origin:||China|
|Minimum Order Quantity:||1 bag|
|Packaging Details:||small package or bulk distribute or OEM|
|Delivery Time:||8work days|
|Payment Terms:||L/C, D/A, D/P, T/T, Western Union, MoneyGram|
|Supply Ability:||100 Bag/Bags Per Day|
|Concentration:||≥ 2000 U/mg||Appearance:||White Powder|
|Group:||In Vitro Diagnostic Products||Recommend Use Concentration:||/|
|Logo Printing:||With Logo Printing||Transport Package:||Packing|
|Production Capacity:||100 Bag/Bags Per Day||Storage Conditions:||Store At -20°C|
1g In Vitro Diagnostic Products,
N9066 In Vitro Diagnostic Products,
DNase I Powder
DNase I Powder
BR Grade, For Research Use Only
Cat. No. N9066
Size: 1 g
Specific Activity: ≥ 2000 U/mg
Form: Contains 1 g DNase I, lyophilized powder
Store at -20°C for 2 years.
DNase I is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA: DNA hybrids.
• Degradation of DNA template in transcription reactions
• DNase I footprinting
• Nick translation
Definition of Activity Unit
One unit of the enzyme completely degrades 1 µg of plasmid DNA in 10 min at 37°C. Enzyme activity is assayed in the following mixture: 40 mM Tris-HCl (pH 8.0), 10 mM MgSO4, 1 mM CaCl2, 1 µg of pUC19 DNA.
38 kDa monomer.
Functionally tested for digestion of template DNA after in vitro transcription.
Inhibition and Inactivation
Inhibitors: metal chelators, transition metals (e.g., Zn) in millimolar concentrations, SDS (even at concentrations less than 0.1%), reducing agents (DTT and beta-mercaptoethanol), ionic strength above 50-100 mM.
Inactivated by heating at 65°C for 10 min in the presence of EGTA or EDTA (use at least 1 mol of EGTA/EDTA per 1 mol of Mn2+/Mg2+).
• DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.
• This grade of DNase I is not used for removing DNA from RNA preparations.
Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.