DNase I Powder N9066 1g In Vitro Diagnostic Products

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: N9066
Minimum Order Quantity: 1 bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: N9066 Size: 1g
Concentration: ≥ 2000 U/mg Appearance: White Powder
Group: In Vitro Diagnostic Products Recommend Use Concentration: /
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
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1g In Vitro Diagnostic Products


N9066 In Vitro Diagnostic Products


DNase I Powder

Product Description

DNase I Powder

BR Grade, For Research Use Only

Cat. No. N9066

Size: 1 g

Specific Activity: ≥ 2000 U/mg

Form: Contains 1 g DNase I, lyophilized powder

Store at -20°C for 2 years.



DNase I is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA: DNA hybrids.



• Degradation of DNA template in transcription reactions

• DNase I footprinting

• Nick translation


Definition of Activity Unit

One unit of the enzyme completely degrades 1 µg of plasmid DNA in 10 min at 37°C. Enzyme activity is assayed in the following mixture: 40 mM Tris-HCl (pH 8.0), 10 mM MgSO4, 1 mM CaCl2, 1 µg of pUC19 DNA.



Bovine pancreas.


Molecular Weight

38 kDa monomer.


Quality Control

Functionally tested for digestion of template DNA after in vitro transcription.


Inhibition and Inactivation

Inhibitors: metal chelators, transition metals (e.g., Zn) in millimolar concentrations, SDS (even at concentrations less than 0.1%), reducing agents (DTT and beta-mercaptoethanol), ionic strength above 50-100 mM.

Inactivated by heating at 65°C for 10 min in the presence of EGTA or EDTA (use at least 1 mol of EGTA/EDTA per 1 mol of Mn2+/Mg2+).



• DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

• This grade of DNase I is not used for removing DNA from RNA preparations.


DNase I Powder N9066 1g In Vitro Diagnostic Products 0

DNase I Powder N9066 1g In Vitro Diagnostic Products 1

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Contact Details

Phone Number : +8618898478663