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V5009 V5010 One Step RT QPCR Master Mix GDSBio RNA Reverse Transcription And Quantitative PCR

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: V5009/V5010
Minimum Order Quantity: 1Bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: V5009/V5010 Specification: 200 Rxn /5000 Rxn
Appearance: Colourless Application: RNA Reverse Transcription And Quantitative PCR
Activity: Pass Amplification Capability: Good
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
High Light:

V5010 RT QPCR Master Mix

,

Quantitative PCR Detection Master Mix

,

One Step Probe RT qPCR Kit V2


Product Description

One-step Probe RT-qPCR Kit V2

High Quality One-Step Probe RT-QPCR Kit V2 V5009/V5010

Cat. No.: V5009,V5010

 

Components

Component V5009 (200 rxn, 30 μl/rxn) V5010 (5000 rxn, 30 μl/rxn)
2X RT-qPCR Mix * 760 μl × 4 38.4 ml × 2
Enzyme Mix b 308 μl 7.7 ml

a. Contains dNTP/dUTP Mix, Mg2+.

b. Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and hot-start Taq DNA Polymerase.

 

Storage

This reagent should be kept at -20°C.

 

Description

The One-step Probe RT-qPCR Kit V2 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Thermostable reverse transcriptase can maintain stable activity at 55°C. The hot-start antibody-modified fast DNA polymerase and optimized buffer system ensure the extremely high sensitivity and specificity of the One Step Probe RT-qPCR Kit V2. The kit is available as a convenient Master Mix. 2 × RT-qPCR Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan®.

 

 

V5009 V5010 One Step RT QPCR Master Mix GDSBio RNA Reverse Transcription And Quantitative PCR 0

V5009 V5010 One Step RT QPCR Master Mix GDSBio RNA Reverse Transcription And Quantitative PCR 1

Product Selection Guide

Cat. No. V5001/V5002 V5005/V5006 V5005L/V5006L V5007/V5008 V5007L/V5008L V5009/V5010 V5011
Product Name One-step Probe RT-qPCR Kit DSPathTM4X One-Step Multiplex Master Mix DSPathTM4X One-Step Multiplex Master Mix (Lyophilized) 2X One Step Prime RT-qPCR Mix 2X One Step Prime RT-qPCR Mix (Lyophilized) One-step Probe RT-qPCR Kit V2 One-step Probe RT-qPCR Kit V3
Mixture Format Enzyme Mix+2X Buffer Mix 4X All-in Mix 4X All-in Lyophilized Powder 2X All-in Mix 2X All-in Lyophilized Powder Enzyme Mix+2X Buffer Mix Enzyme Mix+5X Buffer Mix
Mode/Time Standard/~120 min Fast/~60 min or Standard/~120 min Fast/~60 min or Standard/~120 min Fast/~60 min or Standard/~120 min Fast/~60 min or Standard/~120 min Fast/~60 min or Standard/~88 min Fast/~45min or Standard/~80min
RT Temperature 50~55°C 48~55°C 48~55°C 48~55°C 48~55°C 50~55°C 50~55°C
PCR Enzyme Antibody-modified hotstart Taq Antibody-modified hotstart Taq Antibody-modified hotstart Taq Antibody-modified hotstart Taq Antibody-modified hotstart Taq Antibody-modified hotstart Taq Antibody-modified hotstart Taq
Heat-labile UDG + + + + + + +
Sensitivity Very high Very high Very high Very high Very high Ultra high Ultra high

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

V5009 V5010 One Step RT QPCR Master Mix GDSBio RNA Reverse Transcription And Quantitative PCR 2

V5009 V5010 One Step RT QPCR Master Mix GDSBio RNA Reverse Transcription And Quantitative PCR 3

Contact Details
sales

Phone Number : +8618898478663