RNase-Free Spin Column General RNA Extraction Kit R1051 50 Preps
Product Details:
Place of Origin: | China |
Brand Name: | GDSBio |
Certification: | / |
Model Number: | R1051 |
Payment & Shipping Terms:
Minimum Order Quantity: | 1 box |
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Price: | $9.90 - $49.90/ box |
Packaging Details: | small package or bulk distribute or OEM |
Delivery Time: | 8 work days |
Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
Supply Ability: | 100 Box/Boxes per Day |
Detail Information |
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Catalog No.: | R1051 | Specification: | 50 Preps |
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Logo Printing: | With Logo Printing | Transport Package: | Packing |
Storage Conditions: | This Reagent Should Be Kept At 15-25°C. | ||
High Light: | 50 preps General RNA Extraction Kit,RNase Free General RNA Extraction Kit |
Product Description
General RNA Extraction Kit
Catalog No.: R1051 (50 preps)
Kit Contents
Component | R1051 |
50 preps | |
Solution RL | 60 ml |
Wash Buffer RPI | 18 ml |
Wash Buffer RW | 12 ml |
DEPC-treated water | 10 ml |
RNase-free Spin Column | 50 each |
RNase-free Microcentrifuge Tube | 50 each |
Materials supplied by the users:
• Chloroform
• Ethanol (96–100%)
Description
General RNA Extraction Kit provides a simple method of isolating total RNA from a wide range of sample types and amounts. In general, samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt which is capable of protecting the RNA from endogenous RNases. After homogenization, ethanol is added to the sample. The sample is then processed through a spin column containing a clear silica-based membrane where the RNA binds. Any impurities are effectively removed by subsequent washing. The purified total RNA is then eluted in RNase-Free Water and is suitable for use in a variety of downstream applications.
Downstream Applications
Purified RNA is free of impurities and enzyme inhibitors, and have an A260/280=1.8-2.0, is suitable for applications such as:
• Reverse transcription PCR (RT-PCR)
• Real-time quantitative PCR (qPCR)
• Northern blotting
• Nuclease protection assays
• RNA amplification for microarray analysis
• cDNA library preparation after poly(A)+ selection
Features
• Stable yield
• Reliable performance of high-quality purified total RNA in downstream applications
Storage
DEPC-treated water store at -20 ̊C, other reagents store at 2-8 ̊C, protect from light. Kit contents are stable for up to 12 months when stored properly.
GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, nucleic acid electrophoresis, nucleic acid purification, it has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.