RT-PCR Mix for qPCR
For research use only
GDSBio RT-PCR Mix for qPCR premixed reverse transcription reaction kit R1031
Cat. No.: R1031 Size: 100 rxns (20 µl reaction system)
||R1031 (100 rxns)
|5X RT Buffer
||1 ml × 2
All components should be stored at -20°C, and avoid repeated freezing and thawing.
RT-PCR Mix for qPCR is a premixed reverse transcription reaction (RT-PCR) kit, easy to operate, can quickly and efficiently complete the reverse transcription reaction. Enzyme Mix contains highly efficient reverse transcriptase and heat labile DNase, which can complete genomic DNA clearance and RT-PCR of RNA in one step. RT Buffer contains other components required for RT-PCR, without the need for multiple addition of components step by step, thus reducing the risk of sample contamination. At the same time, the kit is equipped with Control Mix for a negative control reaction to test for genomic DNA residue in the RNA template. The reverse transcription products of this kit have good compatibility for real-time PCR (qPCR) reaction by dye method and probe method, and can be used for accurate gene expression analysis.
First strand cDNA synthesis.
1. This product contains heat labile DNase, so please use each component on ice.
2. To ensure uniform composition, please centrifuge briefly of Enzyme Mix, 5X RT Buffer and Control Mix, then use pipette to blow and mix well before use.
3. During the operation, care should be taken to prevent RNA degradation due to the introduction of RNase. RNA is best stored at -70°C, and avoid repeated freeze-thaw.
4. cDNA products should be stored at -20°C or below, and repeated freeze-thaw should be avoided.
GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, nucleic acid electrophoresis, nucleic acid purification, it has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.