|Place of Origin:||China|
|Minimum Order Quantity:||1Bag|
|Packaging Details:||small package or bulk distribute or OEM|
|Delivery Time:||8 work days|
|Payment Terms:||L/C, D/A, D/P, T/T, Western Union, MoneyGram|
|Supply Ability:||100 Bag/Bags Per Day|
|Cat. No.:||P2101c/P2102c||Specification:||P2101b, 400 Rxn/20μl Reaction P2102b, 2000 Rxn/20μl Reaction|
|Appearance:||Colourless||Group:||Real Time PCR Mix|
|Logo Printing:||With Logo Printing||Transport Package:||Packing|
|Production Capacity:||100 Bag/Bags Per Day||Storage Conditions:||Store At -20°C|
GDSBio master mixture for pcr,
master mixture for pcr ROX,
400 rxn 2x master mix pcr
Cat. No: P2101c, 400 rxn/20μl reaction
P2102c, 2000 rxn/20μl reaction
Power Green qPCR Mix with ROX
For research use only
|2X Power Green qPCR Mix||1 ml||1 ml × 5|
|50X ROX Reference Dye H*||40 μl||200 μl|
|50X ROX Reference Dye L*||40 μl||200 μl|
|Nuclease-free Water||1 ml||1 ml × 5|
* ROX Reference Dye H and ROX Reference Dye L are suitable for fluorescence quantitative PCR instruments requiring high and low concentrations of ROX calibration respectively. It is recommended that 40 μL 50X ROX Reference Dye H or L be premixed into 1ml 2X SYBR Green qPCR Mix. The final working concentration of ROX is 1X.
This mix can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light. The ROX Reference Dye should be kept at -20°C and protected from light.
Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the SYBR Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on.
The Hotstart Taq DNA Polymerase in the mix is chemically modified, and it can reduce non-specific amplification more effectively than the antibody-modified polymerase. At the same time, the mix contains aptamer-based inhibitors, which reduce the generation of the primer dimer and secondary metabolites. When the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This dual hot-start technique brings greater efficiency and specificity to PCR.
• Gene expression analysis
• Low-copy gene detection
• Microarray validation
• Gene knockdown validation
• Compatible with many Real-time systems whether requires ROX reference dye to calibration or not
• Exceptional specificity with dual hot-start mechanism
• Tight reproducibility in Ct values over a broad dynamic range
• Universal instrument compatibility
Table of Instrument Guide
|Instruments||Final Conc. of ROX|
|ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700||500 nM,high ROX|
|ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000||50 nM,low ROX|
|Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler||No ROX|
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.