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Power Green qPCR Mix with ROX for ABI Roche DNA real time PCR
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | P2101c |
Payment & Shipping Terms:
| Minimum Order Quantity: | 1ml |
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| Price: | USD 39~49/ml |
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
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Detail Information |
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| Product Name: | Power Green QPCR Mix With ROX | Specification: | 1 ML |
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| Appearance: | Colourless Solution | Application: | DNA Real Time PCR |
| Storage Conditions: | Store At -20°C Protect From Light | Shipment Location: | Guangzhou, China |
| High Light: | GDSBio master mixture for pcr,master mixture for pcr ROX,400 rxn 2x master mix pcr |
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Product Description
Cat. No: P2101c, 400 rxn/20μl reaction
P2102c, 2000 rxn/20μl reaction
Power Green qPCR Mix with ROX
Power Green qPCR Mix with ROX P2101c/P2102c
For research use only
Components
| Component | P2101c | P2102c |
| 2X Power Green qPCR Mix | 1 ml | 1 ml × 5 |
| 50X ROX Reference Dye H* | 40 μl | 200 μl |
| 50X ROX Reference Dye L* | 40 μl | 200 μl |
| Nuclease-free Water | 1 ml | 1 ml × 5 |
* ROX Reference Dye H and ROX Reference Dye L are suitable for fluorescence quantitative PCR instruments requiring high and low concentrations of ROX calibration respectively. It is recommended that 40 μL 50X ROX Reference Dye H or L be premixed into 1ml 2X SYBR Green qPCR Mix. The final working concentration of ROX is 1X.
Storage
This mix can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light. The ROX Reference Dye should be kept at -20°C and protected from light.
Description
Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the SYBR Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on.
The Hotstart Taq DNA Polymerase in the mix is modified by antibody. When the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This technology helps to reduce non-specific amplification and primer dimer effectively.
The reaction system of this product can be prepared at room temperature without an ice box. The prepared PCR reaction system can be placed at room temperature for 24 hours and the amplification efficiency remains unchanged.
Applications
• Gene expression analysis
• Low-copy gene detection
• Microarray validation
• Gene knockdown validation
Features
• Compatible with many Real-time systems whether requires ROX reference dye to calibration or not
• Exceptional specificity with dual hot-start mechanism
• Tight reproducibility in Ct values over a broad dynamic range
• Universal instrument compatibility
Table of Instrument Guide
| Instruments | Final Conc. of ROX |
| ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700 | 500 nM,high ROX |
| ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000 | 50 nM,low ROX |
| Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler | No ROX |
Testing Data:
Power Green qPCR Mix
Cat. No.: P2101/P2102
The Power Green qPCR Mix utilizes an antibody-modified hot-start Taq DNA polymerase to enhance the specificity of the reaction, ensuring the accuracy of gene quantification analysis. Specially optimized, this product allows for the preparation of the reaction system without the need for an ice box, and can be directly prepared at room temperature with components such as PCR Mix, primers, and templates. The prepared PCR reaction system can be kept at room temperature for 24 hours without changing the amplification efficiency, ensuring the stability of the workbench for large-scale testing.
qPCR Amplification Curve (left) and Melting Curve (right)
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.