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GDSBio Power Green Master Mixture For PCR With ROX P2101c P2102c

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: P2101c/P2102c
Minimum Order Quantity: 1Bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: P2101c/P2102c Specification: P2101b, 400 Rxn/20μl Reaction P2102b, 2000 Rxn/20μl Reaction
Appearance: Colourless Group: Real Time PCR Mix
Activity: Pass Amplification Capability: Good
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
High Light:

GDSBio master mixture for pcr

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master mixture for pcr ROX

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400 rxn 2x master mix pcr


Product Description

Cat. No: P2101c, 400 rxn/20μl reaction

P2102c, 2000 rxn/20μl reaction

 

Power Green qPCR Mix with ROX

Power Green qPCR Mix with ROX P2101c/P2102c

For research use only

Components

Component P2101c P2102c
2X Power Green qPCR Mix 1 ml 1 ml × 5
50X ROX Reference Dye H* 40 μl 200 μl
50X ROX Reference Dye L* 40 μl 200 μl
Nuclease-free Water 1 ml 1 ml × 5

* ROX Reference Dye H and ROX Reference Dye L are suitable for fluorescence quantitative PCR instruments requiring high and low concentrations of ROX calibration respectively. It is recommended that 40 μL 50X ROX Reference Dye H or L be premixed into 1ml 2X SYBR Green qPCR Mix. The final working concentration of ROX is 1X.

 

Storage

This mix can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light. The ROX Reference Dye should be kept at -20°C and protected from light.

 

Description

Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the SYBR Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on.

The Hotstart Taq DNA Polymerase in the mix is chemically modified, and it can reduce non-specific amplification more effectively than the antibody-modified polymerase. At the same time, the mix contains aptamer-based inhibitors, which reduce the generation of the primer dimer and secondary metabolites. When the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This dual hot-start technique brings greater efficiency and specificity to PCR.

 

Applications

• Gene expression analysis

• Low-copy gene detection

• Microarray validation

• Gene knockdown validation

 

Features

• Compatible with many Real-time systems whether requires ROX reference dye to calibration or not

• Exceptional specificity with dual hot-start mechanism

• Tight reproducibility in Ct values over a broad dynamic range

• Universal instrument compatibility

 

Table of Instrument Guide

Instruments Final Conc. of ROX
ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700 500 nM,high ROX
ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000 50 nM,low ROX
Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler No ROX

GDSBio Power Green Master Mixture For PCR With ROX P2101c P2102c 0

GDSBio Power Green Master Mixture For PCR With ROX P2101c P2102c 1

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

GDSBio Power Green Master Mixture For PCR With ROX P2101c P2102c 2

GDSBio Power Green Master Mixture For PCR With ROX P2101c P2102c 3

Contact Details
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Phone Number : +8618898478663